| First Author | Perumal NB | Year | 1997 |
| Journal | J Immunol | Volume | 158 |
| Issue | 12 | Pages | 5744-50 |
| PubMed ID | 9190924 | Mgi Jnum | J:41580 |
| Mgi Id | MGI:894067 | Doi | 10.4049/jimmunol.158.12.5744 |
| Citation | Perumal NB, et al. (1997) TCR-gamma genes are rearranged but not transcribed in IL-7R alpha-deficient mice. J Immunol 158(12):5744-50 |
| abstractText | IL-7, a cytokine produced by bone marrow and thymic stroma, is a growth factor for B and T lymphocytes very early in their development, The IL-7R is a heterodimer of an alpha-chain that specifically binds IL-7 and the common gamma-chain, gamma(c), which is also a component of the receptors for IL-2, IL-4, IL-9, and IL-15, IL-7 has also been hypothesized to play a role in the differentiation of gamma delta T cells, which is supported by the recent findings that mise deficient in the alpha-chain of the IL- 7R (IL-7R alpha-/-) or IL-7 (IL-7-/-) have a complete absence of gamma delta T cells, but not alpha beta T cells, We shaw in this work that V gamma 4 and V gamma 6 TCR genes are rearranged, and sterile V gamma 4 and V gamma 6 TCR-gamma transcripts are expressed in IL-7R-/- -/- thymocytes, but these TCR-gamma genes, and V gamma 5, are not transcribed in thymocytes from IL-7R alpha-/- mice, RAG-1 and RAG-2 genes are transcriptionally active in fetal and adult IL-7R alpha-/- thymocytes, The IL-7- inducible transcription factor, STAT5, is Plot active ia the fetal thymus of IL-7R alpha-/- compared with IL-7R alpha+/+ mice. These data point to a specific role for IL- 7/IL-7R signaling in regulating the transcriptional activity, possibly mediated by SYAT5, of the rearranged TCR-gamma complex during development of gamma delta T cells, and point to mechanistic differences in the regulation of rearrangement of V gamma 4 and V gamma 6 genes vs V gamma 5. |
