| First Author | Lian J | Year | 2020 |
| Journal | Cell Rep | Volume | 31 |
| Issue | 8 | Pages | 107679 |
| PubMed ID | 32460031 | Mgi Jnum | J:304664 |
| Mgi Id | MGI:6514520 | Doi | 10.1016/j.celrep.2020.107679 |
| Citation | Lian J, et al. (2020) Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization. Cell Rep 31(8):107679 |
| abstractText | Generating robust CD4(+) T-helper cell type 1 (Th1) responses is essential for protective vaccine-induced type 1 immunity. Here, we examine whether immunization formulation associated with enhanced vaccine efficacy promotes antigen targeting and cell recruitment into lymph node (LN) niches associated with optimal type 1 responses. Immunization with antigen and Toll-like receptor agonist emulsified in oil leads to an increased differentiation of IFNgamma/TNF-alpha(+) polyfunctional Th1 cells compared to an identical immunization in saline. Oil immunization results in a rapid delivery and persistence of antigen in interfollicular regions (IFRs) of the LN, whereas without oil, antigen is distributed in the medullary region. Following oil immunization, CXCL10-producing inflammatory monocytes accumulate in the IFR, which mobilizes antigen-specific CD4(+) T cells into this niche. In this microenvironment, CD4(+) T cells are advantageously positioned to encounter arriving IL-12-producing inflammatory dendritic cells (DCs). These data suggest that formulations delivering antigen to the LN IFR create an inflammatory niche that can improve vaccine efficacy. |
