| First Author | Kamijo R | Year | 1994 |
| Journal | Science | Volume | 263 |
| Issue | 5153 | Pages | 1612-5 |
| PubMed ID | 7510419 | Mgi Jnum | J:17806 |
| Mgi Id | MGI:65833 | Doi | 10.1126/science.7510419 |
| Citation | Kamijo R, et al. (1994) Requirement for transcription factor IRF-1 in NO synthase induction in macrophages. Science 263(5153):1612-5 |
| abstractText | Production of nitric oxide (NO) by macrophages is important for the killing of intracellular infectious agents. Interferon (IFN)-gamma and lipopolysaccharide stimulate NO production by transcriptionally up-regulating the inducible NO synthase (iNOS). Macrophages from mice with a targeted disruption of the IFN regulatory factor-1 (IRF-1) gene (IRF-1-/- mice) produced little or no NO and synthesized barely detectable iNOS messenger RNA in response to stimulation. Two adjacent IRF-1 response elements were identified in the iNOS promoter. Infection with Mycobacterium bovis (BCG) was more severe in IRF-1-/- mice than in wild-type mice. Thus, IRF-1 is essential for iNOS activation in murine macrophages. |
