| Primary Identifier | MGI:1857222 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ncam1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance cassette replaced most of exons 3 and 4 and the intervening intron. Ribonuclease protection assays did not detect wild-type or read-through transcripts or alternative or cryptic splicing in brain tissue from homozygous mutant mice. Western blot analysis of brain tissue using a monoclonal antibody or a polyclonal rabbit serum did not detect protein in homozygous mutant mice. Similarly, immunostaining did not detect protein in mutant olfactory bulb. |
