| First Author | Patterson M | Year | 2014 |
| Journal | J Virol | Volume | 88 |
| Issue | 4 | Pages | 1914-23 |
| PubMed ID | 24284323 | Mgi Jnum | J:205274 |
| Mgi Id | MGI:5544511 | Doi | 10.1128/JVI.02925-13 |
| Citation | Patterson M, et al. (2014) Rescue of a Recombinant Machupo Virus from Cloned cDNAs and In Vivo Characterization in Interferon (alphabeta/gamma) Receptor Double Knockout Mice. J Virol 88(4):1914-23 |
| abstractText | Machupo virus (MACV) is the etiological agent of Bolivian hemorrhagic fever (BHF), a reemerging and neglected tropical disease associated with high mortality. The prototypical strain of MACV, Carvallo, was isolated from a human patient in 1963, but minimal in vitro and in vivo characterization has been reported. To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned cDNAs. The recombinant MACV (rMACV) had in vitro growth properties similar to those of the parental MACV. Both viruses caused similar disease development in alpha/beta and gamma interferon receptor knockout mice, including neurological disease development and high mortality. In addition, we have identified a novel murine model with mortality and neurological disease similar to BHF disease reported in humans and nonhuman primates. |
