| Primary Identifier | MGI:1857303 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Apob |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | Levels of LDL-cholesterol, VLDL, and triglyceride LDL are lower relative to wild-type mice in APOB48 only mice in the presence of wild-type APOE production(J:33830). APOB48 only mice have higher levels of cholesterol and more atherosclerotic lesions than APOB100 only mice in the absence of APOE (J:41510). |
| molecularNote | A "hit and run"-type vector was used to create a stop signal in codon 2179 (codon 2153 in the original publication) in sequences corresponding to the apo-B48 editing codon. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB48 isoform is unaffected by this mutation, while no ApoB100 isoform is produced from this allele. |
