| First Author | Fan Z | Year | 2010 |
| Journal | Nat Med | Volume | 16 |
| Issue | 6 | Pages | 718-22 |
| PubMed ID | 20495571 | Mgi Jnum | J:161525 |
| Mgi Id | MGI:4459576 | Doi | 10.1038/nm.2155 |
| Citation | Fan Z, et al. (2010) In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response. Nat Med 16(6):718-22 |
| abstractText | Here we present methods to longitudinally track islet allograft-infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (T(eff) cells) and discrimination between natural and induced regulatory T cells (nT(reg) and iT(reg) cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance. |
