| First Author | Zhao H | Year | 2020 |
| Journal | Nucleic Acids Res | Volume | 48 |
| Issue | 17 | Pages | 9621-9636 |
| PubMed ID | 32853367 | Mgi Jnum | J:296383 |
| Mgi Id | MGI:6459809 | Doi | 10.1093/nar/gkaa711 |
| Citation | Zhao H, et al. (2020) A role of the CTCF binding site at enhancer Ealpha in the dynamic chromatin organization of the Tcra-Tcrd locus. Nucleic Acids Res 48(17):9621-9636 |
| abstractText | The regulation of T cell receptor Tcra gene rearrangement has been extensively studied. The enhancer Ealpha plays an essential role in Tcra rearrangement by establishing a recombination centre in the Jalpha array and a chromatin hub for interactions between Valpha and Jalpha genes. But the mechanism of the Ealpha and its downstream CTCF binding site (here named EACBE) in dynamic chromatin regulation is unknown. The Hi-C data showed that the EACBE is located at the sub-TAD boundary which separates the Tcra-Tcrd locus and the downstream region including the Dad1 gene. The EACBE is required for long-distance regulation of the Ealpha on the proximal Valpha genes, and its deletion impaired the Tcra rearrangement. We also noticed that the EACBE and Ealpha regulate the genes in the downstream sub-TAD via asymmetric chromatin extrusion. This study provides a new insight into the role of CTCF binding sites at TAD boundaries in gene regulation. |
