| First Author | Sanyal M | Year | 2009 |
| Journal | Int Immunol | Volume | 21 |
| Issue | 11 | Pages | 1225-37 |
| PubMed ID | 19737782 | Mgi Jnum | J:154186 |
| Mgi Id | MGI:4367395 | Doi | 10.1093/intimm/dxp090 |
| Citation | Sanyal M, et al. (2009) Enhanced B cell activation in the absence of CD81. Int Immunol 21(11):1225-37 |
| abstractText | CD81 is a component of the CD19/CD21 co-receptor complex in B cells. However, the role of CD81 in B cell activation has not been clearly elucidated. Here, we demonstrate that Cd81(-/-) B cells stimulated via their B cell receptor fluxed higher intracellular-free calcium ion along with increased phosphorylation of spleen tyrosine kinase and phospholipase gamma 2. Additionally, Cd81(-/-) B cells responded to toll like receptor 4 stimulation with increased nuclear factor-kappa B activation, cell proliferation and antibody secretion compared with wild-type B cells. Cd81(-/-) mice also mounted a significantly higher immune response to T-independent antigens than their wild-type counterparts. Finally, analysis of Cd81(-/-) B cells that were generated by bone marrow transplantation into Rag1(-/-) mice confirmed that the hyperactive phenotype is not dependent on the CD81-deficient environment. Taken together, these results indicate that CD81 plays a negative role in B cell activation in vitro and in vivo. |
