| First Author | Barajas-Mora EM | Year | 2019 |
| Journal | Mol Cell | Volume | 73 |
| Issue | 1 | Pages | 48-60.e5 |
| PubMed ID | 30449725 | Mgi Jnum | J:273872 |
| Mgi Id | MGI:6282809 | Doi | 10.1016/j.molcel.2018.10.013 |
| Citation | Barajas-Mora EM, et al. (2019) A B-Cell-Specific Enhancer Orchestrates Nuclear Architecture to Generate a Diverse Antigen Receptor Repertoire. Mol Cell 73(1):48-60.e5 |
| abstractText | The genome is organized into topologically associated domains (TADs) that enclose smaller subTADs. Here, we identify and characterize an enhancer that is located in the middle of the V gene region of the immunoglobulin kappa light chain (Igkappa) locus that becomes active preceding the stage at which this locus undergoes V(D)J recombination. This enhancer is a hub of long-range chromatin interactions connecting subTADs in the V gene region with the recombination center at the J genes. Deletion of this element results in a highly altered long-range chromatin interaction pattern across the locus and, importantly, affects individual V gene utilization locus-wide. These results indicate the existence of an enhancer-dependent framework in the Igkappa locus and further suggest that the composition of the diverse antibody repertoire is regulated in a subTAD-specific manner. This enhancer thus plays a structural role in orchestrating the proper folding of the Igkappa locus in preparation for V(D)J recombination. |
