| First Author | Düber S | Year | 2009 |
| Journal | Blood | Volume | 114 |
| Issue | 24 | Pages | 4960-7 |
| PubMed ID | 19812384 | Mgi Jnum | J:155473 |
| Mgi Id | MGI:4414590 | Doi | 10.1182/blood-2009-04-218156 |
| Citation | Duber S, et al. (2009) Induction of B-cell development in adult mice reveals the ability of bone marrow to produce B-1a cells. Blood 114(24):4960-7 |
| abstractText | To study B-cell development from bone marrow (BM), we generated recombination-activating gene 1 (Rag1)-targeted mice lacking mature lymphocytes. B-cell development can be induced in such mice by B cell-specific restoration of a functional Rag1 transcription unit. Follicular and marginal zone B cells populated the spleen when Rag1 expression was permitted. Notably, the peritoneal cavity was dominated by bona fide B-1a cells, as judged by surface markers and functional properties. These BM-derived B-1a cells exhibited a polyclonal VDJ repertoire with substantial N nucleotide insertions. Nevertheless, physiologic frequencies of phosphatidylcholine-specific B cells were detected. Importantly, the BM of young and 5-month-old mice was indistinguishable with regard to the potential to generate B-1a cells. |
