| First Author | Takase M | Year | 2013 |
| Journal | Int Immunol | Volume | 25 |
| Issue | 3 | Pages | 145-56 |
| PubMed ID | 23042789 | Mgi Jnum | J:193293 |
| Mgi Id | MGI:5468083 | Doi | 10.1093/intimm/dxs095 |
| Citation | Takase M, et al. (2013) Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development. Int Immunol 25(3):145-56 |
| abstractText | The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged immunoglobulin heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface immunoglobulin, while the B1-cell maintenance requires the peritoneal environment. The B1-cell expansion takes place in spleen, and is held in check by competitor B2 cells. The results indicate that the NT-ES method could replace the transgenic or knock-in mouse approaches currently used to study the biology of cells that undergo somatic rearrangements of their antigen receptor genes. |
