| First Author | Grover HS | Year | 2014 |
| Journal | Cell Rep | Volume | 7 |
| Issue | 5 | Pages | 1716-1728 |
| PubMed ID | 24857659 | Mgi Jnum | J:277937 |
| Mgi Id | MGI:6274125 | Doi | 10.1016/j.celrep.2014.04.031 |
| Citation | Grover HS, et al. (2014) Impact of regulated secretion on antiparasitic CD8 T cell responses. Cell Rep 7(5):1716-1728 |
| abstractText | CD8 T cells play a key role in defense against the intracellular parasite Toxoplasma, but why certain CD8 responses are more potent than others is not well understood. Here, we describe a parasite antigen, ROP5, that elicits a CD8 T cell response in genetically susceptible mice. ROP5 is secreted via parasite organelles termed rhoptries that are injected directly into host cells during invasion, whereas the protective, dense-granule antigen GRA6 is constitutively secreted into the parasitophorous vacuole. Transgenic parasites in which the ROP5 antigenic epitope was targeted for secretion through dense granules led to enhanced CD8 T cell responses, whereas targeting the GRA6 epitope to rhoptries led to reduced CD8 responses. CD8 T cell responses to the dense-granule-targeted ROP5 epitope resulted in reduced parasite load in the brain. These data suggest that the mode of secretion affects the efficacy of parasite-specific CD8 T cell responses. |
