| First Author | Lilić M | Year | 2002 |
| Journal | J Immunol | Volume | 169 |
| Issue | 9 | Pages | 4945-50 |
| PubMed ID | 12391207 | Mgi Jnum | J:79811 |
| Mgi Id | MGI:2388962 | Doi | 10.4049/jimmunol.169.9.4945 |
| Citation | Lilic M, et al. (2002) The role of fibroblasts in thymocyte-positive selection. J Immunol 169(9):4945-50 |
| abstractText | Mice with fibroblast-specific expression of TAP-1 were generated by expressing the TAP-1 transgene under the control of the fibroblast-specific protein (FSP) 1 promoter/enhancer on TAP-1-deficient background. MHC class I expression in primary fibroblast cultures isolated from the resulting strain mimicked that of wild-type counterparts. MHC class I was detected in both types of fibroblasts following treatment with IFN-alphabeta. Positive selection of CD4(-)CD8(+) thymocytes was observed in neither adult nor fetal/neonatal thymus of transgenic mice. IFN-alphabeta-induced expression of MHC class I rescued positive selection of CD4(-)CD8(+) T cells in fetal thymic organ cultures, but not in adult mice. Contrary to previous suggestions, our results indicate a limited role of fibroblasts in promoting positive selection. In addition, the results suggest that positive selection may occur by a different mechanism in fetal vs adult thymus. |
