| First Author | Gigante ED | Year | 2018 |
| Journal | Dev Biol | Volume | 437 |
| Issue | 2 | Pages | 152-162 |
| PubMed ID | 29571613 | Mgi Jnum | J:262122 |
| Mgi Id | MGI:6158036 | Doi | 10.1016/j.ydbio.2018.03.019 |
| Citation | Gigante ED, et al. (2018) Hypomorphic Smo mutant with inefficient ciliary enrichment disrupts the highest level of vertebrate Hedgehog response. Dev Biol 437(2):152-162 |
| abstractText | Smoothened (Smo) is the essential transducer of Sonic hedgehog (Shh) signaling, which regulates cell fate and proliferation during embryogenesis. We identified a novel mouse mutant, cabbie (cbb), and found that its cause is a missense mutation in Smo. We showed the Smo(cbb) mutation is insensitive to the Shh agonist SAG, perhaps due to the disruption of SAG binding. We characterized Smo(cbb) for defects in craniofacial and skeletal development, as well as neural tube patterning, and revealed Smo(cbb) affected processes that require the highest levels of Shh activity. Smo is normally enriched in cilia upon Shh stimulation; however, we detected inefficient enrichment of Smo in Smo(cbb) mutants whether we stimulated with Shh or SAG. Taken together, our data suggest that the highest levels of vertebrate Hedgehog signaling activity require efficient Smo ciliary enrichment. |
