| First Author | Mahendroo M | Year | 2004 |
| Journal | Mol Cell Endocrinol | Volume | 222 |
| Issue | 1-2 | Pages | 113-20 |
| PubMed ID | 15249131 | Mgi Jnum | J:91120 |
| Mgi Id | MGI:3045995 | Doi | 10.1016/j.mce.2004.04.009 |
| Citation | Mahendroo M, et al. (2004) Steroid 5alpha-reductase 1 promotes 5alpha-androstane-3alpha,17beta-diol synthesis in immature mouse testes by two pathways. Mol Cell Endocrinol 222(1-2):113-20 |
| abstractText | 5alpha-Androstane-3alpha,17beta-diol (androstanediol) is the predominant androgen in immature mouse testes, and studies were designed to investigate its pathway of synthesis, the steroid 5alpha-reductase isoenzyme involved in its formation, and whether testicular androstanediol is formed in embryonic mouse testes at the time of male phenotypic development. In 24-26-day-old immature testes, androstanediol is formed by two pathways; the predominant one involves testosterone --> dihydrotestosterone --> androstanediol, and a second utilizes the pathway progesterone --> 5alpha-dihydroprogesterone --> 5alpha-pregnane-3alpha-ol-20-one --> 5alpha-pregnane-3alpha,17alpha-diol-20-one --> androsterone --> androstanediol. Formation of androstanediol was normal in testes from mice deficient in steroid 5alpha-reductase 2 but absent in testes from mice deficient in steroid 5alpha-reductase 1, indicating that isoenzyme 2 is not expressed in day 24-26 testes. The fact that androstenedione and testosterone were the only androgens identified after incubation of day 16 and 17 embryonic testes with [ [Formula: see text] ]progesterone implies that androstanediol formation in the testis plays no role in male phenotypic differentiation in the mouse. |
