| First Author | Rubino SJ | Year | 2013 |
| Journal | Eur J Immunol | Volume | 43 |
| Issue | 11 | Pages | 2896-906 |
| PubMed ID | 23881368 | Mgi Jnum | J:203009 |
| Mgi Id | MGI:5523756 | Doi | 10.1002/eji.201243028 |
| Citation | Rubino SJ, et al. (2013) Constitutive induction of intestinal Tc17 cells in the absence of hematopoietic cell-specific MHC class II expression. Eur J Immunol 43(11):2896-906 |
| abstractText | The enteric pathogen Citrobacter rodentium induces a mucosal IL-17 response in CD4(+) T helper (Th17) cells that is dependent on the Nod-like receptors Nod1 and Nod2. Here, we sought to determine whether this early Th17 response required antigen presentation by major histocompatibility complex class II (MHCII) for full induction. At early phases of C. rodentium infection, we observed that the intestinal mucosal Th17 response was fully blunted in irradiated mice reconstituted with MHCII-deficient (MHCII(-/-) -->WT) hematopoietic cells. Surprisingly, we also observed a substantial increase in the relative frequency of IL-17(+) CD8(+) CD4(-) TCR-beta(+) cells (Tc17 cells) and FOXP3(+) CD8(+) CD4(-) TCR-beta(+) cells in the lamina propria and intraepithelial lymphocyte compartment of MHCII(-/-) -->WT mice compared with that in WT-->WT counterparts. Moreover, MHCII(-/-) -->WT mice displayed increased susceptibility, increased bacterial translocation to deeper organs, and more severe colonic histopathology after infection with C. rodentium. Finally, a similar phenotype was observed in mice deficient for CIITA, a transcriptional regulator of MHCII expression. Together, these results indicate that MHCII is required to mount early mucosal Th17 responses to an enteric pathogen, and that MHCII regulates the induction of atypical CD8(+) T-cell subsets, such as Tc17 cells and FOXP3(+) CD8(+) cells, in vivo. |
