| First Author | Johnson BL 3rd | Year | 2013 |
| Journal | Biochem Biophys Res Commun | Volume | 437 |
| Issue | 4 | Pages | 591-6 |
| PubMed ID | 23850678 | Mgi Jnum | J:210776 |
| Mgi Id | MGI:5571817 | Doi | 10.1016/j.bbrc.2013.06.118 |
| Citation | Johnson BL 3rd, et al. (2013) Mechanisms underlying mouse TNF-alpha stimulated neutrophil derived microparticle generation. Biochem Biophys Res Commun 437(4):591-6 |
| abstractText | Despite advances in understanding and treatment of sepsis, it remains a disease with high mortality. Neutrophil Derived Microparticles (NDMPs) are present during sepsis and can modulate the immune system. As TNF-alpha is a cytokine that predominates in the initial stages of sepsis, we evaluated whether and how TNF-alpha can induce NDMPs in mice. We observed that TNF-alpha treatment results in increased NDMP numbers. We also determined that the activation of either TNF receptor 1 (TNFr1) or TNF receptor 2 (TNFr2) resulted in increased NDMP numbers and that activation of both resulted in an additive increase. Inhibition of Caspase 8 diminishes NDMPs generated through TNFr1 activation and inhibition of NF-kappaB abrogates NDMPs generated through activation of both TNFr1 and TNFr2. We conclude that the early production of TNF-alpha during sepsis can increase NDMP numbers through activation of the Caspase 8 pathway or NF-kappaB. |
