| First Author | Navarro P | Year | 1995 |
| Journal | Mol Immunol | Volume | 32 |
| Issue | 1 | Pages | 1-8 |
| PubMed ID | 7870054 | Mgi Jnum | J:23278 |
| Mgi Id | MGI:71044 | Doi | 10.1016/0161-5890(94)00143-o |
| Citation | Navarro P, et al. (1995) The complete cDNA and deduced amino acid sequence of equine IgE. Mol Immunol 32(1):1-8 |
| abstractText | The cDNA from a transcript encoding the complete heavy chain of the equine immunoglobulin IgE has been cloned and sequenced. A fragment of the equine epsilon gene was amplified from cDNA using PCR and this fragment was then used to probe a horse cDNA library prepared from peripheral blood lymphocytes. A recombinant clone containing the cDNA encoding the complete horse epsilon chain and its associated V-D-J and leader, was subsequently isolated and sequenced. Comparison of the deduced amino acid sequence of equine IgE with the C epsilon heavy chains of other species indicates it to be most similar to human C epsilon (54%), followed by sheep C epsilon (52%); the greatest sequence similarity was found in the C epsilon 3 and C epsilon 4 domains among the species compared. The associated V-region had greatest similarity in FR1 to those reported for sheep and the cow (> 80%) but less than 60% to the VH sequences of other species in FR3. |
