| First Author | Williams DR Jr | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 334 |
| Issue | 3 | Pages | 960-6 |
| PubMed ID | 16039617 | Mgi Jnum | J:100032 |
| Mgi Id | MGI:3586407 | Doi | 10.1016/j.bbrc.2005.06.189 |
| Citation | Williams DR Jr, et al. (2005) Limb chondrogenesis is compromised in the versican deficient hdf mouse. Biochem Biophys Res Commun 334(3):960-6 |
| abstractText | It has been suggested that the matrix proteoglycan, versican, may perform a functional role during early events of limb skeletogenesis largely by virtue of its spatiotemporal expression pattern in precartilage mesenchymal aggregations. The versican-deficient hdf transgenic mouse has provided the first model to explore the implications of a null mature versican on limb chondrogenesis. Due to lethality of hdf homozygous embryos prior to limb cartilage differentiation, high-density micromass cultures were employed to compare the chondrogenic capacity of hdf mutant limb mesenchyme to that of wild-type. In homozygous hdf mesenchyme, aggregation was severely compromised and neither cartilage-characteristic Type II collagen nor alcian blue positive foci were detected during a 6-day period of culture. Three-dimensional culture of hdf mutant mesenchyme, however, showed that in a permissive environment mutant cells also expressed Type II collagen. Results strongly suggest that mature versican proteoglycan is essential for precartilage aggregation and subsequent cartilage differentiation. |
