| First Author | Apte RN | Year | 1977 |
| Journal | Exp Hematol | Volume | 5 |
| Issue | Suppl 2 | Pages | 12 (Abstr.) |
| Mgi Jnum | J:30692 | Mgi Id | MGI:78275 |
| Citation | Apte RN, et al. (1977) Relationship between lipopolysaccharide-induced serum colony stimulating factor and interferon in regulation of murine granulopoiesis. Exp Hematol 5(Suppl 2):12 (Abstr.) |
| abstractText | Full text of Abstract. RELATIONSHIP BETWEEN LIPOPOLYSACCHARIDE-INDUCED SERUM COLONY STIMULATING FACTOR AND INTERFERON IN REGULATION OF MURINE GRANULOPOIESIS. R.N. Apte, O. Ascher and D.H. Pluznik. Dept. of Life Sciences, Bar-llan University, Ramat-Gan, Israel. Administration of bacterial lipopolysaccharide (LPS) to mice causes a rise in tissue and serum colony stimulating factor (CSF) levels as well as an elevation in serum interferon (IF). In addition, the time course of appearance of both mediators in the serum is almost identical. Previous evidence has shown that IF antagonizes the action of CSF, by inhibiting the formation of granulocyte/macrophage derived from hemopoietic committed stem cells (colony forming cells Ð CFC) in soft agar cultures. A genetic experimental model was used in order to study the mutual relationship between both mediators. Two inbred strains of mice differing in the response to LPS were used to study the genetic control of generation of CSF and IF: a high responder strain (C3H/eB), which generates acute levels of CSF and IF, and a low responder strain (C3H/HeJ), which fails to show these responses. It was found that the ability to generate serum CSF, as well as IF, is controlled by single autosomal dominant genes. The associated relationships of CSF and IF responsiveness have been investigated in backcross (F1 x C3H/HeJ) and F2 mice. Most mice which generated high levels of CSF showed a high IF response, while low responsiveness in generations of CSF was also paralleled by low responsiveness of IF secretion. Our results indicate that both LPS-induced mediators are associated and controlled by similar genetic mechanisms. In order to elucidate the mutual relationship between the above-mentioned factors in regulation of granulopoiesis various quantitative methods have to be used to ascertain whether IF antagonizes CSF. |
