| First Author | Gounder AP | Year | 2016 |
| Journal | PLoS Pathog | Volume | 12 |
| Issue | 3 | Pages | e1005474 |
| PubMed ID | 26933888 | Mgi Jnum | J:250598 |
| Mgi Id | MGI:5918132 | Doi | 10.1371/journal.ppat.1005474 |
| Citation | Gounder AP, et al. (2016) Defensins Potentiate a Neutralizing Antibody Response to Enteric Viral Infection. PLoS Pathog 12(3):e1005474 |
| abstractText | alpha-defensins are abundant antimicrobial peptides with broad, potent antibacterial, antifungal, and antiviral activities in vitro. Although their contribution to host defense against bacteria in vivo has been demonstrated, comparable studies of their antiviral activity in vivo are lacking. Using a mouse model deficient in activated alpha-defensins in the small intestine, we show that Paneth cell alpha-defensins protect mice from oral infection by a pathogenic virus, mouse adenovirus 1 (MAdV-1). Survival differences between mouse genotypes are lost upon parenteral MAdV-1 infection, strongly implicating a role for intestinal defenses in attenuating pathogenesis. Although differences in alpha-defensin expression impact the composition of the ileal commensal bacterial population, depletion studies using broad-spectrum antibiotics revealed no effect of the microbiota on alpha-defensin-dependent viral pathogenesis. Moreover, despite the sensitivity of MAdV-1 infection to alpha-defensin neutralization in cell culture, we observed no barrier effect due to Paneth cell alpha-defensin activation on the kinetics and magnitude of MAdV-1 dissemination to the brain. Rather, a protective neutralizing antibody response was delayed in the absence of alpha-defensins. This effect was specific to oral viral infection, because antibody responses to parenteral or mucosal ovalbumin exposure were not affected by alpha-defensin deficiency. Thus, alpha-defensins play an important role as adjuvants in antiviral immunity in vivo that is distinct from their direct antiviral activity observed in cell culture. |
