| First Author | Qin J | Year | 2002 |
| Journal | J Hered | Volume | 93 |
| Issue | 3 | Pages | 201-5 |
| PubMed ID | 12195036 | Mgi Jnum | J:102776 |
| Mgi Id | MGI:3608059 | Doi | 10.1093/jhered/93.3.201 |
| Citation | Qin J, et al. (2002) Evidence for the lack of mismatch-repair directed antirecombination during mouse meiosis. J Hered 93(3):201-5 |
| abstractText | Meiotic recombination was studied in DNA mismatch repair (MMR)-deficient mice using a strain carrying a Pms2 knockout mutation. Using single-sperm typing, recombination was analyzed over five intervals on four chromosomes in four Pms2 -/- animals. A total of 1936 meioses were studied and compared to 1848 meioses from three Pms2 +/+ controls. A smaller study was carried out on a single interval in each of two chromosomes in an MMR-deficient mouse homozygous for the Msh2 knockout mutation. A total of 792 meioses were examined in the Msh2 -/- and 880 meioses in the Msh2 +/+ animal. Recombination fractions were not significantly different in either of the MMR-deficient mouse strains when compared to MMR-proficient controls. Our results appear to conflict with mouse embryonic stem (ES) cell gene-targeting experiments where MMR plays a major role in determining the efficiency of homologous recombination between nonidentical sequences. A number of possibilities could explain the apparent lack of a significant effect on meiosis. |
