| Primary Identifier | MGI:1857797 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gli1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance cassette replaced exons 9 to 11, which encode part of zinc finger 1, zinc fingers 2 - 5, and 43 adjacent amino acids. Northern blots of total RNA from E13.5 embryos using a cDNA probe 3' to the zinc fingers resulted in detection of 2 transcripts in wild-type embryos and only one smaller broad band in homozygous mutant embryos. Further analysis by RT-PCR followed by sequencing of the products showed that the mutant allele produced a transcript that spliced around the neomycin insert from exon 8 - 12. The authors state that as a result, the mutant allele could not encode for a protein with DNA-binding activity, but could produce a mutant protein lacking amino acids 269 - 437. |
