| First Author | Wang X | Year | 2006 |
| Journal | J Mol Cell Cardiol | Volume | 40 |
| Issue | 4 | Pages | 503-9 |
| PubMed ID | 16519896 | Mgi Jnum | J:108903 |
| Mgi Id | MGI:3625252 | Doi | 10.1016/j.yjmcc.2006.01.005 |
| Citation | Wang X, et al. (2006) A role for muscle LIM protein (MLP) in vascular remodeling. J Mol Cell Cardiol 40(4):503-9 |
| abstractText | Given the well-defined role of LIM-motif containing proteins in cytoskeletal organization, cell fate, and differentiation, we hypothesized that the regulation of LIM proteins played an integral role in vascular remodeling. We screened a compendium of cDNA microarray data from rat vascular smooth muscle cells (VSMC) for novel LIM-containing targets and identified muscle LIM protein (MLP), a gene previously thought to be only in striated muscle. Sequence analysis, RTQPCR and Western blotting reconfirmed expression of MLP in VSMC. MLP was elevated>10-fold 7 days following balloon injury in the rat carotid artery. Wire injury led to a significantly increased intima/media ratio in MLP -/- mice compared to wild-type controls (P<0.007, N=5). Fas-ligand and ceramide-induced apoptosis were significantly decreased in MLP deficient VSMC (n=6, P<0.001). Adenoviral-induced restoration of MLP significantly restored apoptotic response (N=6, P<0.001). These findings are the first to identify MLP in vascular smooth muscle and demonstrate that it plays a critical role in vascular remodeling. This is consistent with earlier findings demonstrating a role for MLP in striated muscle remodeling in response to load and stretch. |
