| First Author | Ha A | Year | 2012 |
| Journal | Mol Vis | Volume | 18 |
| Pages | 645-56 | PubMed ID | 22509096 |
| Mgi Jnum | J:191619 | Mgi Id | MGI:5462170 |
| Citation | Ha A, et al. (2012) Identification of Wnt/beta-catenin modulated genes in the developing retina. Mol Vis 18:645-56 |
| abstractText | PURPOSE: During mammalian eye development, the restriction of Wnt/beta-catenin signaling at the junction of the neural retina and the retinal pigment epithelium in the peripheral eyecup is required for the development of the ciliary margin, a non-neural region of the eyecup that is the precursor of the ciliary body and iris of the adult eye. METHODS: To identify genes that are modulated by beta-catenin activity in the embryonic retina, we performed gene expression profiling in Li(+)-treated retinal explants, a pharmacological model of beta-catenin activation. The Li(+)-modulated gene data set was searched for beta-catenin/T-cell specific transcription factor binding sites. RESULTS: Functional annotations of this data set revealed significant enrichments for genes involved in chromatin organization, neurogenesis, and cell motion/migration. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis confirmed the modulation of 12 genes in Li(+)-treated explants and retinas of mice with Cre-mediated induction of constitutively active beta-catenin (beta-cat(act)). In situ hybridization revealed beta-catenin-specific upregulation of cyclin-dependent kinase inhibitor 1A (P21) [Cdkn1a] and tumor necrosis factor receptor superfamily, member 19 (Tnfrsf19) in the developing retina consistent with the antineurogenic and proliferation changes associated with ectopic Wnt/beta-catenin signaling in the eyecup. CONCLUSIONS: This data set of Li(+)-modulated genes provides a valuable resource for characterizing the Wnt/ beta-catenin regulated gene network in eyecup patterning. |
