| First Author | Duffy EB | Year | 2016 |
| Journal | J Leukoc Biol | Volume | 100 |
| Issue | 6 | Pages | 1335-1347 |
| PubMed ID | 27365531 | Mgi Jnum | J:325821 |
| Mgi Id | MGI:6873387 | Doi | 10.1189/jlb.2A1215-555RR |
| Citation | Duffy EB, et al. (2016) FcgammaR mediates TLR2- and Syk-dependent NLRP3 inflammasome activation by inactivated Francisella tularensis LVS immune complexes. J Leukoc Biol 100(6):1335-1347 |
| abstractText | IgG (mAb)-opsonized, inactivated Francisella tularensis LVS (iFt-mAb) enhances TLR2-dependent IL-6 production by macrophages via Fcgamma receptors (FcgammaR). In mice, vaccination with iFt-mAb provides IgA-dependent protection against lethal challenge with Ft LVS. Because inflammasome maturation of IL-1beta is thought important for antibody-mediated immunity, we considered the possibility that iFt-mAb elicits an FcgammaR-dependent myeloid cell inflammasome response. Herein, we find that iFt-mAb enhances macrophage and dendritic cell IL-1beta responses in a TLR2- and FcgammaR-dependent fashion. Although iFt-mAb complexes bind FcgammaR and are internalized, sensing of cytosolic DNA by absent in melanoma 2 (AIM2) is not required for the IL-1beta response. In contrast, ASC, caspase-1, and NLR family pyrin domain-containing 3 (NLRP3) are indispensable. Further, FcgammaR-mediated spleen tyrosine kinase (Syk) signaling is required for this NLRP3-dependent IL-1beta response, but the alternative IL-1beta convertase caspase-8 is insufficient. Finally, iFt-mAb-vaccinated wild-type mice exhibit a significant delay in time to death, but IL-1R1- or Nlrp3-deficient mice vaccinated in this way are not protected and lack appreciable Francisella-specific antibodies. This study demonstrates that FcgammaR-mediated Syk activation leads to NLRP3 inflammasome-dependent IL-1beta production in macrophages and suggests that an Nlrp3- and IL-1R-dependent process contributes to the IgA response important for protection against Ft LVS. These findings extend our understanding of cellular responses to inactivated pathogen-opsonized vaccine, establish FcgammaR-elicited Syk kinase-mediated NLRP3 inflammasome activation, and provide additional insight toward understanding crosstalk between TLR and FcgammaR signals. |
