| First Author | Shen A | Year | 2018 |
| Journal | Nat Commun | Volume | 9 |
| Issue | 1 | Pages | 1050 |
| PubMed ID | 29535304 | Mgi Jnum | J:260750 |
| Mgi Id | MGI:6149445 | Doi | 10.1038/s41467-018-03459-7 |
| Citation | Shen A, et al. (2018) Functionally distinct and selectively phosphorylated GPCR subpopulations co-exist in a single cell. Nat Commun 9(1):1050 |
| abstractText | G protein-coupled receptors (GPCRs) transduce pleiotropic intracellular signals in a broad range of physiological responses and disease states. Activated GPCRs can undergo agonist-induced phosphorylation by G protein receptor kinases (GRKs) and second messenger-dependent protein kinases such as protein kinase A (PKA). Here, we characterize spatially segregated subpopulations of beta2-adrenergic receptor (beta2AR) undergoing selective phosphorylation by GRKs or PKA in a single cell. GRKs primarily label monomeric beta2ARs that undergo endocytosis, whereas PKA modifies dimeric beta2ARs that remain at the cell surface. In hippocampal neurons, PKA-phosphorylated beta2ARs are enriched in dendrites, whereas GRK-phosphorylated beta2ARs accumulate in soma, being excluded from dendrites in a neuron maturation-dependent manner. Moreover, we show that PKA-phosphorylated beta2ARs are necessary to augment the activity of L-type calcium channel. Collectively, these findings provide evidence that functionally distinct subpopulations of this prototypical GPCR exist in a single cell. |
