| First Author | Sharma J | Year | 2011 |
| Journal | Cell Immunol | Volume | 269 |
| Issue | 2 | Pages | 69-73 |
| PubMed ID | 21497800 | Mgi Jnum | J:172279 |
| Mgi Id | MGI:5006871 | Doi | 10.1016/j.cellimm.2011.03.023 |
| Citation | Sharma J, et al. (2011) TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu. Cell Immunol 269(2):69-73 |
| abstractText | The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains. |
