| First Author | Cascalho M | Year | 1999 |
| Journal | Dev Immunol | Volume | 7 |
| Issue | 1 | Pages | 43-50 |
| PubMed ID | 10636478 | Mgi Jnum | J:134556 |
| Mgi Id | MGI:3789248 | Doi | 10.1155/1999/24514 |
| Citation | Cascalho M, et al. (1999) A mouse with a monoclonal primary immunoglobulin repertoire not further diversified by V-gene replacement. Dev Immunol 7(1):43-50 |
| abstractText | We have generated a monoclonal B-cell mouse by introducing homozygous, nonfunctional RAG-2 alleles and a lambda1 light-chain transgene into the quasi-monoclonal (QM) mouse, which contains a 'knocked-in' V(H)DJ(H) rearrangement. Thus, this mouse, which we call MonoB, is devoid of T cells and contains preformed heavy- and light-chain genes encoding immunoglobulin with an anti-NP specificity. The MonoB mouse allows us to examine immunoglobulin diversity in the absence of processes mediated by V(D)J recombination and T cells. Here we report that not only is the MonoB's primary immunoglobulin repertoire monoclonal, but also that its secondary repertoire is not further diversified by V-gene replacement or gene conversion. Among 99 heavy-chain and 41 lambda light-chain genes from peripheral B cells of the MonoB mouse, there were no V-gene replacements. When compared to the QM mouse, which has RAG activity, and for which V-gene replacement is the major diversifying mechanism, these data suggest that V-gene replacement is mediated by V(D)J recombination and not by other recombination systems. |
