| First Author | Hawwari A | Year | 2005 |
| Journal | Nat Immunol | Volume | 6 |
| Issue | 5 | Pages | 481-9 |
| PubMed ID | 15806105 | Mgi Jnum | J:97736 |
| Mgi Id | MGI:3576188 | Doi | 10.1038/ni1189 |
| Citation | Hawwari A, et al. (2005) Regulation of T cell receptor alpha gene assembly by a complex hierarchy of germline Jalpha promoters. Nat Immunol 6(5):481-9 |
| abstractText | Assembly of the gene encoding T cell receptor alpha (Tcra) is characterized by an orderly progression of primary and secondary V(alpha)-to-J(alpha) recombination events across the J(alpha) array, but the targeting mechanisms responsible for this progression are mostly unknown. Studies have shown that the T early-alpha promoter is important in targeting primary Tcra rearrangements. We found that T early-alpha and a previously unknown promoter associated with J(alpha)49 targeted primary recombination to discrete sets of constant alpha region (C(alpha))-distal J(alpha) segments and together directed nearly all normal primary recombination events. Furthermore, deletion of the T early-alpha promoter activated previously suppressed downstream promoters and stimulated primary rearrangement to centrally located J(alpha) segments. Central promoter derepression also occurred after primary rearrangement, thereby providing a mechanism to target secondary recombination events. |
