| First Author | Villey I | Year | 1996 |
| Journal | Immunity | Volume | 5 |
| Issue | 4 | Pages | 331-42 |
| PubMed ID | 8885866 | Mgi Jnum | J:77518 |
| Mgi Id | MGI:2181918 | Doi | 10.1016/s1074-7613(00)80259-9 |
| Citation | Villey I, et al. (1996) Defect in rearrangement of the most 5' TCR-J alpha following targeted deletion of T early alpha (TEA): implications for TCR alpha locus accessibility. Immunity 5(4):331-42 |
| abstractText | To address the role of the TEA germline transcription, which initiates upstream of the TCR-J alpha S, in the regulation of TCR-J alpha locus accessibility, we created a mouse in which this region has been removed by homologous recombination. Normal development of T alpha beta cells and the expression of other TCR alpha germline transcripts in TEA-/- mice ruled out an exclusive role for TEA in the overall accessibility of the J alpha cluster. However, the rearrangement of the most 5' J alpha (J alpha 61 to J alpha 53) was severely impaired, indicating that TEA may control the DNA accessibility of a particular J alpha window. Moreover, the relative usage of every J alpha segment was affected. These results are consistent with TEA acting as a 'rearrangement-focusing' element, targeting the primary waves of V alpha-J alpha recombination to the most 5' J alpha S in an ongoing TCR-J alpha rearrangement model. |
