| First Author | Okamoto M | Year | 2023 |
| Journal | Cell Rep | Volume | 42 |
| Issue | 7 | Pages | 112813 |
| PubMed ID | 37440410 | Mgi Jnum | J:338342 |
| Mgi Id | MGI:7511287 | Doi | 10.1016/j.celrep.2023.112813 |
| Citation | Okamoto M, et al. (2023) A genetic method specifically delineates Th1-type Treg cells and their roles in tumor immunity. Cell Rep 42(7):112813 |
| abstractText | Regulatory T (Treg) cells expressing the transcription factor (TF) Foxp3 also express other TFs shared by T helper (Th) subsets under certain conditions. Here, to determine the roles of T-bet-expressing Treg cells, we generate a mouse strain, called VeDTR, in which T-bet/Foxp3 double-positive cells are engineered to be specifically labeled and depleted by a combination of Cre- and Flp-recombinase-dependent gene expression control. Characterization of T-bet(+)Foxp3(+) cells using VeDTR mice reveals high resistance under oxidative stress, which is involved in accumulation of T-bet(+)Foxp3(+) cells in tumor tissues. Moreover, short-term depletion of T-bet(+)Foxp3(+) cells leads to anti-tumor immunity but not autoimmunity, whereas that of whole Treg cells does both. Although ablation of T-bet(+)Foxp3(+) cells during Toxoplasma infection slightly enhances Th1 immune responses, it does not affect the course of the infection. Collectively, the intersectional genetic method reveals the specific roles of T-bet(+)Foxp3(+) cells in suppressing tumor immunity. |
