| Primary Identifier | MGI:1859153 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ift88 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| description | Homozygous mutant mice display early to mid-gestation lethality (~embryonic day 9.5), random patterning of the left-right body axis, neural tube defects, pericardial sac expansion, enlarged limb buds, and absent embryonic node cilia. |
| molecularNote | A genomic fragment containing exons 2 and part of 3 was replaced with a lacZ gene with a splice acceptor site and neomycin selection cassette. RT-PCR analysis demonstrated that the mutation eliminated expression of the wild-type transcript, and whole-mount embryo staining studies revealed that beta-galactosidase is expressed from this allele. |
