| First Author | Abbott C | Year | 2003 |
| Journal | MGI Direct Data Submission | Mgi Jnum | J:86481 |
| Mgi Id | MGI:2680184 | Citation | Abbott C, et al. (2003) Mapping of the Dancer mutation on Chromosome 19. MGI Direct Data Submission |
| abstractText | The aim of the project was to use microsatellite markers to map the locus of the spontaneous mutation Dancer (Dc) on chromosome 19. Dancer mice (C3H/J) were obtained from The Jackson Laboratory and the breeding stock was maintained within this C3H/J background. Heterozygous Dc mice exhibit head-tossing and circling behaviour, and are easily identifiable from normal C3H/J wildtypes. Although the Dc+/- mice are viable, we found that the fertility of some of these mice was reduced. Dancer homozygotes are born with cleft lip and cleft palate, and do not survive after birth. We found that homozygous mice were difficult to generate because matings of circling Dc+/- males and females were usually unsuccessful or the litter sizes were reduced in comparison to wildtype matings. Initially, a backcross was set up using C57 mice, but the resulting N2 mice did not have a fully penetrant circling phenotype, thus it was difficult to differentiate between Dc+/- and wildtype mice. Therefore, a backcross using CBA mice was set up. After an initial mating between a circling Dc+/- (C3H/J) male and a wildtype CBA female, only circling Dc+/- F1 mice were selected to be backcrossed to a CBA wildtype mouse, and the resulting circling N2 mice were genotyped. |
