| First Author | Kondoh D | Year | 2014 |
| Journal | J Biol Chem | Volume | 289 |
| Issue | 52 | Pages | 36158-65 |
| PubMed ID | 25384980 | Mgi Jnum | J:319124 |
| Mgi Id | MGI:6862810 | Doi | 10.1074/jbc.M114.571141 |
| Citation | Kondoh D, et al. (2014) Molecular clock regulates daily alpha1-2-fucosylation of the neural cell adhesion molecule (NCAM) within mouse secondary olfactory neurons. J Biol Chem 289(52):36158-65 |
| abstractText | The circadian clock regulates various behavioral and physiological rhythms in mammals. Circadian changes in olfactory functions such as neuronal firing in the olfactory bulb (OB) and olfactory sensitivity have recently been identified, although the underlying molecular mechanisms remain unknown. We analyzed the temporal profiles of glycan structures in the mouse OB using a high-density microarray that includes 96 lectins, because glycoconjugates play important roles in the nervous system such as neurite outgrowth and synaptogenesis. Sixteen lectin signals significantly fluctuated in the OB, and the intensity of all three that had high affinity for alpha1-2-fucose (alpha1-2Fuc) glycan in the microarray was higher during the nighttime. Histochemical analysis revealed that alpha1-2Fuc glycan is located in a diurnal manner in the lateral olfactory tract that comprises axon bundles of secondary olfactory neurons. The amount of alpha1-2Fuc glycan associated with the major target glycoprotein neural cell adhesion molecule (NCAM) varied in a diurnal fashion, although the mRNA and protein expression of Ncam1 did not. The mRNA and protein expression of Fut1, a alpha1-2-specific fucosyltransferase gene, was diurnal in the OB. Daily fluctuation of the alpha1-2Fuc glycan was obviously damped in homozygous Clock mutant mice with disrupted diurnal Fut1 expression, suggesting that the molecular clock governs rhythmic alpha1-2-fucosylation in secondary olfactory neurons. These findings suggest the possibility that the molecular clock is involved in the diurnal regulation of olfaction via alpha1-2-fucosylation in the olfactory system. |
