| First Author | Hossain FMA | Year | 2020 |
| Journal | Cell Immunol | Volume | 351 |
| Pages | 104066 | PubMed ID | 32089258 |
| Mgi Jnum | J:297865 | Mgi Id | MGI:6479348 |
| Doi | 10.1016/j.cellimm.2020.104066 | Citation | Hossain FMA, et al. (2020) CCR5 attenuates neutrophilic airway inflammation exacerbated by infection with rhinovirus. Cell Immunol 351:104066 |
| abstractText | Human rhinovirus (hRV) is the most common cause of asthma exacerbation characterized by clinical and pathophysiological heterogeneity. Steroid-sensitive, Th2 type-eosinophilic asthma has been somewhat studied, but hRV-induced neutrophilic asthma exacerbation is poorly understood. Here, CCR5 was found to play a role in attenuating neutrophilic airway inflammation in hRV-induced asthma exacerbation using chicken ovalbumin (OVA)-based model. CCR5 deficiency resulted in exacerbated neutrophilic asthmatic responses in airways following hRV infection. CCR5-deficient mice showed enhanced mucus expression and altered expression of tight junction proteins in lung tissues. CCR5-deficient mice were also manifested with influx of CD45(+)CD11b(+)Siglec-F(+)Gr-1(+) neutrophils, along with enhanced production of IL-17A, IFN-gamma, IL-6, IL-1beta cytokines in inflamed tissues. In contrast, CCR5-deficient mice elicited down-regulation of Th2-related cytokine proteins following hRV infection. More interestingly, the lack of CCR5 altered the equilibrium of CD4(+)FoxP3(+) Tregs and IL-17(+)CD4(+) Th17 in inflamed tissues. CCR5-deficient mice showed increased frequency and absolute number of IL-17-producing CD4(+) Th17 cells in lung tissues compared to wild-type mice, whereas the reduced infiltration of CD4(+)FoxP3(+) Treg cells was observed. CCR5 deficiency resulted in the skewed production of Th17 and Th1 cytokines in lymph nodes and lungs upon OVA stimulation. Likewise, CCR5-deficient mice showed enhanced expression of Th17-inducing cytokines (IL-1beta, IL-6, and TNF-alpha) in lung tissues. These results imply that CCR5 deficiency facilitates Th17 airway inflammation during hRV-induced asthma exacerbation, along with suppressing Th2 responses. Furthermore, our results suggest that CCR5 attenuates hRV-induced neutrophilic airway inflammation through conserving the equilibrium of CD4(+)Foxp3(+) Treg cells and IL-17(+)CD4(+) Th17 cells in hRV-induced asthma exacerbation. |
