| First Author | Janssen MJ | Year | 2009 |
| Journal | J Neurosci | Volume | 29 |
| Issue | 16 | Pages | 5116-26 |
| PubMed ID | 19386907 | Mgi Jnum | J:252956 |
| Mgi Id | MGI:6100189 | Doi | 10.1523/JNEUROSCI.4737-08.2009 |
| Citation | Janssen MJ, et al. (2009) Dopamine modulation of GABA tonic conductance in striatal output neurons. J Neurosci 29(16):5116-26 |
| abstractText | We previously reported greater GABAA receptor-mediated tonic currents in D2+ striatopallidal than D1+ striatonigral medium spiny neurons (MSNs) are mediated by alpha5-subunit-containing receptors. Here, we used whole-cell recordings in slices from bacterial artificial chromosome transgenic mice to investigate the link between subunit composition, phosphorylation, and dopamine receptor activation. Whole-cell recordings in slices from delta-subunit knock-out mice demonstrate that while MSNs in wild-type mice do express delta-subunit-containing receptors, this receptor subtype is not responsible for tonic conductance observed in the acute slice preparation. We assessed the contribution of the beta1- and beta3-subunits expressed in MSNs by their sensitivity to etomidate, an agonist selective for beta2- or beta3-subunit-containing GABAA receptors. Although etomidate produced substantial tonic current in D2+ neurons, there was no effect in D1+ neurons. However, with internal PKA application or dopamine modulation, D1+ neurons expressed tonic conductance and responded to etomidate application. Our results suggest that distinct phosphorylation of beta3-subunits may cause larger tonic current in D2+ striatopallidal MSNs, and proper intracellular conditions can reveal tonic current in D1+ cells. |
