| First Author | Yoshioka R | Year | 2010 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 51 |
| Issue | 11 | Pages | 5630-9 |
| PubMed ID | 20554614 | Mgi Jnum | J:171398 |
| Mgi Id | MGI:4949827 | Doi | 10.1167/iovs.10-5158 |
| Citation | Yoshioka R, et al. (2010) Corneal epithelial wound healing impaired in keratinocyte-specific HB-EGF-deficient mice in vivo and in vitro. Invest Ophthalmol Vis Sci 51(11):5630-9 |
| abstractText | PURPOSE: To study the role played by HB-EGF in corneal epithelial wound healing. METHODS: A 2-mm corneal epithelial wound was created in keratinocyte-specific, HB-EGF-deficient mice--HB(lox/lox):K-5Cre (HB(-/-))--and the speed of wound healing was compared with that in wild-type (WT) mice. Cultured confluent mouse corneal epithelial cells (MCECs) from WT and HB(-/-) mice were scraped, and the bare area was measured. The proliferation of MCECs was determined by BrdU incorporation. The degree of attachment of WT and HB(-/-) MCECs was also determined. The mRNA expression of EGF family and cell adhesion molecules was determined by real-time PCR. RESULTS: Corneal epithelial wound healing was significantly delayed in HB(-/-) mice, and the expression of HB-EGF was detected at the leading edge of the wound in HB(lox/+):K5-Cre (HB(+/-)) mice by the presence of lacZ staining. The wound closure was significantly impaired in HB(-/-) MCECs and was improved by adding HB-EGF. The number of BrdU-positive MCECs of WT and HB(-/-) mice was not significantly different, and both increased to different degrees when HB-EGF was added. The adhesion of isolated HB(-/-) MCECs was lower than that of WT MCECs, but the degree of adhesion was restored by adding HB-EGF. The expression of epiregulin was upregulated in HB(-/-) MCECs, and alpha6- and beta1-integrin were upregulated by adding HB-EGF. CONCLUSIONS: HB-EGF plays an important role in corneal epithelial cell healing by enhancing cellular attachment and part of cell proliferation. |
