| First Author | Sun F | Year | 2015 |
| Journal | Sci Rep | Volume | 5 |
| Pages | 17594 | PubMed ID | 26621350 |
| Mgi Jnum | J:251222 | Mgi Id | MGI:6101610 |
| Doi | 10.1038/srep17594 | Citation | Sun F, et al. (2015) Id4 Marks Spermatogonial Stem Cells in the Mouse Testis. Sci Rep 5:17594 |
| abstractText | Mammalian spermatogenesis is a classic adult stems cell-dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing (Id4(+)) cells at different time points in situ and track their behaviors across distinct developmental stages during steady-state and regenerating spermatogenesis. We found that Id4(+) cells continue to produce spermatogonia, spermatocytes and sperm in mouse testis, showing they are capable of self-renewal and have differentiation potential. Consistent with these findings, ablation of Id4(+) cells in mice results in a loss of spermatogenesis. Furthermore, developmental fate mapping reveals that Id4(+) SSCs originate from neonate Id4(+) gonocytes. Therefore, our results indicate that Id4 marks spermatogonial stem cells in the mouse testis. |
