| First Author | Wendling O | Year | 2009 |
| Journal | Genesis | Volume | 47 |
| Issue | 1 | Pages | 14-8 |
| PubMed ID | 18942088 | Mgi Jnum | J:144642 |
| Mgi Id | MGI:3831461 | Doi | 10.1002/dvg.20448 |
| Citation | Wendling O, et al. (2009) Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse. Genesis 47(1):14-8 |
| abstractText | To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER(T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). In this transgenic mouse line, Cre-ER(T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. Moreover, with the exception of few cardiomyocytes, LoxP-flanked DNA excision is restricted to smooth muscle cells. Thus, SMA-Cre-ER(T2) mice should be of great value to analyze gene function in smooth muscles, and to establish new animal models of human smooth muscle disorders. |
