| First Author | Li WL | Year | 2005 |
| Journal | Yi Chuan Xue Bao | Volume | 32 |
| Issue | 9 | Pages | 909-15 |
| PubMed ID | 16201233 | Mgi Jnum | J:161504 |
| Mgi Id | MGI:4459404 | Citation | Li WL, et al. (2005) Endothelial cell-specific expression of Cre recombinase in transgenic mice. Yi Chuan Xue Bao 32(9):909-15 |
| abstractText | Endothelial cells participate in angiogenesis, vascular homeostasis, thrombosis, inflammation and vascular wall remodeling. To study the function of genes in endothelial cells using Cre-loxP system, we generated Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by Tie2 promoter. Total six founder mice carrying the Tie2-Cre transgene were identified by genomic PCR and Southern blot. The integration efficiency is 11%. In order to test the excision activity and tissue distribution of the Cre recombinase, the Tie2-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4(Co/Co)) or the reporter line ROSA26. PCR of multiple tissue DNA from Tie2-Cre; Smad4(Co/+) mice revealed the Cre activity in all tissues containing endothelial cells. We detected pan-endothelial expression of the Cre transgene in Tie2-Cre; ROSA26 double transgenic embryos by lacZ staining. Therefore, this mouse line may serve as a valuable tool for endothelial cell lineage analyses and conditional gene ablation in endothelial cells. |
