| First Author | Müsch W | Year | 2008 |
| Journal | Genesis | Volume | 46 |
| Issue | 3 | Pages | 163-6 |
| PubMed ID | 18327770 | Mgi Jnum | J:133175 |
| Mgi Id | MGI:3777892 | Doi | 10.1002/dvg.20378 |
| Citation | Musch W, et al. (2008) Generation and characterization of alpha-chymase-Cre transgenic mice. Genesis 46(3):163-6 |
| abstractText | The Cre-loxP technology allows the introduction of somatic gene alterations in a tissue and/or cell type specific manner. The development of transgenes that target Cre expression to specific cell types is a critical component in this system. Here, we describe the generation and characterization of transgenic mouse lines expressing Cre recombinase under the control of the baboon alpha-chymase promoter, designated Chm:Cre, in order to direct Cre expression specifically to mouse mast cells. Chm:Cre expression was detected in mast cells in lung and colon tissue. Cre-mediated recombination in these mice identified a population of mature tissue resident mast cells using ROSA26R reporter mice. No Cre-expression and Cre-mediated recombination was induced in in vitro generated bone marrow derived mast cells or mast cells isolated from the peritoneal cavity indicating that Cre-expression under the control of the alpha-chymase promoter is solely activated in tissue resident mast cells. These Chm:Cre transgenic mice represent a useful tool to specifically inactivate genes of interest in mast cells of these tissues. |
