| First Author | Pohlkamp T | Year | 2014 |
| Journal | PLoS One | Volume | 9 |
| Issue | 6 | Pages | e100384 |
| PubMed ID | 24950299 | Mgi Jnum | J:210655 |
| Mgi Id | MGI:5571605 | Doi | 10.1371/journal.pone.0100384 |
| Citation | Pohlkamp T, et al. (2014) Generation and Characterization of an Nse-CreERT2 Transgenic Line Suitable for Inducible Gene Manipulation in Cerebellar Granule Cells. PLoS One 9(6):e100384 |
| abstractText | We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo. |
