|  Help  |  About  |  Contact Us

Publication : Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre.

First Author  Gallardo T Year  2007
Journal  Genesis Volume  45
Issue  6 Pages  413-7
PubMed ID  17551945 Mgi Jnum  J:125051
Mgi Id  MGI:3723401 Doi  10.1002/dvg.20310
Citation  Gallardo T, et al. (2007) Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre. Genesis 45(6):413-7
abstractText  Cell type-specific genetic modification using the Cre/loxP system is a powerful tool for genetic analysis of distinct cell lineages. Because of the exquisite specificity of Vasa expression (confined to the germ cell lineage in invertebrate and vertebrate species), we hypothesized that a Vasa promoter-driven transgenic Cre line would prove useful for the germ cell lineage-specific inactivation of genes. Here we describe a transgenic mouse line, Vasa-Cre, where Cre is efficiently and specifically expressed in germ cells. Northern analysis showed that transgene expression was confined to the gonads. Cre-mediated recombination with the Rosa26-lacZ reporter was observed beginning at approximately e15, and was >95% efficient in male and female germ cells by birth. Although there was a potent maternal effect with some animals showing more widespread recombination, there was no ectopic activity in most adults. This Vasa-Cre transgenic line should thus prove useful for genetic analysis of diverse aspects of gametogenesis and as a general deletor line.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

12 Bio Entities

0 Expression