| First Author | Zhang XM | Year | 2004 |
| Journal | Genesis | Volume | 40 |
| Issue | 1 | Pages | 45-51 |
| PubMed ID | 15354293 | Mgi Jnum | J:92300 |
| Mgi Id | MGI:3052367 | Doi | 10.1002/gene.20062 |
| Citation | Zhang XM, et al. (2004) Highly restricted expression of Cre recombinase in cerebellar Purkinje cells. Genesis 40(1):45-51 |
| abstractText | The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. genesis 40:45-51, 2004. Copyright 2004 Wiley-Liss, Inc. |
