| First Author | Delmas V | Year | 2003 |
| Journal | Genesis | Volume | 36 |
| Issue | 2 | Pages | 73-80 |
| PubMed ID | 12820167 | Mgi Jnum | J:84391 |
| Mgi Id | MGI:2667520 | Doi | 10.1002/gene.10197 |
| Citation | Delmas V, et al. (2003) Cre-mediated recombination in the skin melanocyte lineage. Genesis 36(2):73-80 |
| abstractText | Organ-specific expression of a Cre recombinase allows the analysis of gene function in a particular tissue or cell type. Using a 6.1 kb promoter from the mouse tyrosinase gene, we generated and characterized two lines of transgenic mice that express Cre recombinase in melanoblasts. Utilizing a Cre-responsive reporter mouse strain, genetic recombination was detected in the melanoblasts of the skin from embryonic day 11.5. In addition, Cre-expression was detected in the skin and eyes of mice. Cre transgene activity was occasionally detected in the brain and peripheral nerves but not in other tissues. When Tyr::Cre mice were crossed with mice carrying a homozygous loxP conditional mutation for the insulin-like growth factor receptor gene (Igf1r), Cre-melanoblast-specific recombination pattern was confirmed and no abnormal phenotype was observed. In conclusion, Tyr::Cre transgenic mice provide a valuable tool to follow the cell lineage and to examine gene function in melanocyte development and transformation. |
