| First Author | Ebert SN | Year | 2004 |
| Journal | Dev Dyn | Volume | 231 |
| Issue | 4 | Pages | 849-58 |
| PubMed ID | 15517585 | Mgi Jnum | J:93831 |
| Mgi Id | MGI:3505819 | Doi | 10.1002/dvdy.20188 |
| Citation | Ebert SN, et al. (2004) Targeted insertion of the Cre-recombinase gene at the phenylethanolamine n-methyltransferase locus: A new model for studying the developmental distribution of adrenergic cells. Dev Dyn 231(4):849-58 |
| abstractText | To evaluate the developmental distribution of adrenergic cells in vivo, we inserted the Cre-recombinase gene into the locus encoding for the epinephrine biosynthetic enzyme phenylethanolamine n-methyltransferase (Pnmt) and crossed these Pnmt-Cre mice with ROSA26 reporter (R26R) mice to activate LacZ (encoding beta-galactosidase) expression in cells that were selectively derived from the adrenergic lineage. Our data show the following: (1) Insertion of Cre-recombinase into the Pnmt locus created a functional knockout of Pnmt expression with concomitant loss of epinephrine in homozygous Pnmt(Cre/Cre) mice; (2) Despite the reduction in Pnmt expression and epinephrine production in Pnmt(Cre/Cre) mice, these mice were viable and fertile, with no apparent developmental defects; (3) When crossed with R26R mice, Pnmt-Cre activation of LacZ expression faithfully recapitulated Pnmt expression in vivo; and (4) LacZ expression was activated in substantial numbers of pacemaking, conduction, and working cardiomyocytes. Developmental Dynamics 231:849-858, 2004. (c) 2004 Wiley-Liss, Inc. |
