| First Author | Peterson KR | Year | 2004 |
| Journal | Genesis | Volume | 39 |
| Issue | 1 | Pages | 1-9 |
| PubMed ID | 15124222 | Mgi Jnum | J:89725 |
| Mgi Id | MGI:3041333 | Doi | 10.1002/gene.20020 |
| Citation | Peterson KR, et al. (2004) Transgenic Cre expression mice for generation of erythroid-specific gene alterations. Genesis 39(1):1-9 |
| abstractText | Transgenic mice that express Cre recombinase in erythroid cell lineages were developed so that genes affecting erythropoiesis/hematopoiesis may be altered without necessarily affecting fetus viability. A micro-LCR cassette-beta-globin promoter-Cre recombinase gene (microLCR-betapr-Cre) construct was synthesized and used to generate transgenic mice. Concurrently, we produced mice containing a microLCR-loxP-flanked beta sickle gene (microLCR-loxP-beta(S)-loxP) construct. microLCR-betapr-Cre mice with intact transgenes in variable copy number were identified. Cre expression was assessed by RNAse protection and RT-PCR. Cre function was ascertained by breeding to microLCR-loxP-beta(S)-loxP mice. We demonstrate that beta(S) expression was not detected in the blood of bigenics, but the gene was present in nonerythroid cells. Thus, excision of the loxP-flanked beta(S) gene was restricted to erythroid cell lineages. |
