| First Author | Jullien N | Year | 2008 |
| Journal | Genesis | Volume | 46 |
| Issue | 4 | Pages | 193-9 |
| PubMed ID | 18395834 | Mgi Jnum | J:135150 |
| Mgi Id | MGI:3790533 | Doi | 10.1002/dvg.20383 |
| Citation | Jullien N, et al. (2008) Use of ERT2-iCre-ERT2 for conditional transgenesis. Genesis 46(4):193-9 |
| abstractText | We examined the use of ERT2-iCre-ERT2 (Cre2ERT2), a tamoxifen-regulated form of Cre that has been described to have a background activity lower than that of other tamoxifen-regulated Cre constructs, for establishing performant conditional deleter mouse lines. Cre2ERT2 was inserted by homologous recombination into the Rosa26 locus. These mice were mated with R26R Cre-reporter mice. No recombination could be observed in the progenies in the absence of tamoxifen treatment. Tamoxifen treatment at E13-14 led to a high level, albeit variable, recombination in most of the tissues examined: liver, heart, kidney, brain, lung etc. Treatment of adult animals also induced recombination in these tissues, although at a lower level. Northern blot and qPCR studies suggested that these differences are not linked to significant variations of the level of expression of Cre2ERT2. Thus, Cre2ERT2 appears to be a good alternative to existing modulatable Cre systems, displaying a lack of background activity and a high-level inducibility in vivo. |
